Reporter

Part:BBa_J61032:Design

Designed by: John Anderson   Group: Arkin Lab   (2006-11-10)


Alkaline phosphatase [phoA]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 310
    Illegal NgoMIV site found at 757
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

 pre-pcr ca1033F/ca1032R on Citrobacter gen         (1500 bp)

 PCR ca1032F/R on above PCR                         (1500 bp, XbaI/EcoRI)

 Sub into pSB1A2-Bca1008                            (XbaI/MfeI, 2077+935, large)

 Product is pSB1A2-Bca1032

 ca1032F	Forward oligo to biobrick phoA of Citrobacter

 GGTTAtctagaTGTTTTTTATTTTAACTGTATTTATACATGGAG

 ca1032R	Reverse oligo to biobrick phoA of Citrobacter

 cagtaGAATTCccagaggcggcaTTACTTCAG

 ca1033F	Forward constant oligo to clone phoA

 ttatcatcgctccgcatcctg


References